Z374210

Sigma

 

Bioconjugate Techniques

ISBN-10:0-1234-2336-8
ISBN-13:978-0-1234-2336-8

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Description

General descriptionA practical guide to modification and cross-linking technology for research, diagnostics and therapeutics. Contains detailed information on the chemistry, reagent systems and practical applications for creating labeled or conjugated molecules.

Properties

publication infoG. Hermanson, ed., Academic Press, 1996, 785 pp., soft cover

Table Of Contents

Table of ContentsBioconjugate Chemistry: Functional Targets.
The Chemistry of Reactive Groups.
Bioconjugate Reagents: Zero-Length Cross-linkers.
Homobifunctional Cross-linkers.
Heterobifunctional Cross-linkers.
Trifunctional Cross-linkers.
Cleavable Reagent Systems.
Tags and Probes.
Bioconjugate Applications: Preparation of Hapten–Carrier Immunogen Conjugates.
Antibody Modification and Conjugation.
Immunotoxin Conjugation Techniques.
Preparation of Liposome Conjugates and Derivatives.
Avidin-Biotin Systems.
Preparation of Colloidal-Gold-Labeled Proteins.
Modification with Synthetic Polymers.
Enzyme Modification and Conjugation.
Nucleic Acid and Oligonucleotide Modification and Conjugation.
Subject Index.